Biochemistry I Laboratory

CHEM4401

Syllabus (Fall 2011)

Links

Example Lab Report .pdf

Please Note: All "Dry" laboratory assignments must be performed during your laboratory period. Assignments performed outside of lab will not be accepted.

Please Remember: pants, close-toed shoes, safety goggles and lab coat

Please also turn in a "receipt" showing that you have completed the online laboratory safety course

 

Labs and Lab supplements:

Lab 1 - Units, Concentrations, Solutions & Dilutions PDF

Lab 2 - Pipetting & Scales PDF

Lab 3 - Buffers & pH (supplement - Practical Buffer Preparation) PDF

Lab 4 - Photometry. Please also bring your practical buffer prep supplement PDF

Lab 5 - Chromatographic Methods of Amino Acid Separation PDF

Lab 6 - Protein Structure Analysis Please bring your laptop/iPad to Lab PDF

Lab 7 - Analysis of Polyphenoloxidase Enzyme Activity PDF

Lab 8 - Enzyme Kinetics Please bring your data from Lab 7 and a laptop or USB memory drive PDF

Lab 9 - Protein Quantification .Laboratory mid-term to be held in lab. PDF

Lab 10 - Glucose Determination PDF

Lab 11 - Nucleic Acid Purification & Quantification PDF

Lab 12 - Chromatographic Lipid Separation ALL LABS CANCELLED THIS WEEK.

Lab 13 - Laboratory Clean up, Check out, Evaluations and Final Exam

Links:

Dr. Feig's IU bioinformatics website

BLAST Course

BAST Tutorial

National Center For Biotechnology Information (NCBI)

NCBI BLAST search web page

Expert Protein Analysis System (ExPASy)

Kyoto Encyclopedia of Genes and Genomes (KEGG) database

Protein Explorer

BRENDA protein kinetics database

Protein Data bank

ClustalW Protein Sequence Alignment tool

Format Converter (conversion of sequences for ConSurf analysis)

The Sequence Manipulation Suite

Webcutter

 PDF files require that your computer have Adobe Acrobat Reader software installed. It is available for download free by following the link below  

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